Design validated plasmid constructs in plain English.
Describe your construct in a conversation — PlasmidStudio generates annotated, validated plasmid maps in seconds.
Now in beta. Free during beta.
See it in action
Design a GFP expression vector with CMV promoter and kanamycin resistance
I've designed a 5.2 kb construct with CMV promoter, GFP CDS, BGH polyA, and KanR on ColE1 backbone.
Add a His-tag to the C-terminus
Done — 6xHis (18 bp) at position 2,847 bp. Found EcoRI + BamHI sites in the GFP CDS. Remove them silently?
Design Health
- Reading frame intact
- Kozak sequence detected
- Optimal for mammalian expression
This is a real interaction from PlasmidStudio, not a mockup.
Try it yourselfMore than a design tool
AI Lab Protocols
Generate step-by-step wet lab protocols from your construct design. Materials, procedure, primer sequences, and verification strategy — exported as PDF.
Cloning Strategy Advisor
Not sure which cloning method to use? The AI analyzes your construct and recommends Restriction Enzyme, Gibson, Golden Gate, or Gateway.
Design Health Panel
14 automated checks catch missing terminators, frameshift errors, and incompatible origins — with one-click fixes for each issue.
Codon Optimization
Optimize coding sequences for E. coli, HEK293, CHO, yeast, or insect cells. CAI scoring with 100% amino acid preservation.
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Primer Tm Calculator
Nearest-neighbor Tm with polymerase presets, hairpin/dimer analysis, and construct-aware primer regions — no signup required.
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Restriction Enzyme Calculator
Find compatible enzyme pairs for directional cloning. Analyze two sequences, simulate gel patterns, and check methylation sensitivity.
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20 Starter Templates
Mammalian expression, bacterial, CRISPR/Cas9, lentiviral, yeast, Gateway — start from a curated backbone, then modify with AI.
Sequence Verification
Import Sanger or nanopore sequencing results and align against the designed construct. Mismatches highlighted on the plasmid map.
Works with your existing workflow
Import
- GenBank (.gb, .gbk)
- FASTA
- SnapGene (.dna)
- Addgene search
Design
- Restriction Enzyme
- Gibson Assembly
- Golden Gate Assembly
- Gateway Cloning
Export
- GenBank
- FASTA
- SnapGene (.dna)
- SBOL v3
- PDF maps
- IDT / Twist / GenScript
Addgene integration — Browse and import from Addgene's 170,000+ plasmid repository directly from PlasmidStudio.
Missing your cloning method or file format? Tell us what you need
Your sequences stay yours.
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Encrypted. Data encrypted in transit and at rest.
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Private. AI requests processed via API — not used to train models. Providers may briefly retain requests for abuse monitoring.
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Yours. Delete your constructs at any time.
Your constructs are stored in a secure cloud database so you can access them across sessions. AI design requests are routed through our API proxy and are not retained beyond the request.
Learn more about securityJoin the beta — it's free
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Building PlasmidStudio for you.
We're in beta and actively building based on user feedback. Missing a cloning method? Need a file format? Have an idea?
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